In many cases, DNA is isolated from cell cultures or from microorganisms and subsequently used as a PCR template. If, on the other hand, the amount of template is too high, the probability of primers annealing to other (not one hundred percent complimentary) sequences, as well as the formation of primer dimers, will increase, which will result in the amplification of by-products. The higher the number of cycles, the more prevalent the amplification of flawed product will be. A Taq polymerase that is used for most PCR experiments does not feature a correction function (3'-5' exonuclease activity) thus, errors occurring during amplification cannot be corrected. If very little template is used, a corresponding increase in the number of amplification cycles will be needed to obtain a sufficient amount of product.
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